hotbars pixels in texture pack dissapear/misplaced in game , doesnt happen in the image editor

im using a texture pack and the pixels of the texture pack's hot bar don't load correctly. they either are not there at all, or are misplaced. the texture image itself looks completly pixel perfect when i open the image in something like paint.net i really like this texture pack so i would like to get it fixed if its possible. this only happens when i use large gui scale, which is the best size for my monitor.